Science Laboratory Technology  ·  Level 6
Science Laboratory Research

Chapter 4: Prepare for science laboratory research

📚 9 Topics

Compiled by: Kevin Rotich

4.1 Introduction to research methods

Research is a planned, systematic investigation that answers a defined question by collecting and analysing evidence. A laboratory technician meets research in many forms: validating a new method before it replaces an old one, finding why a batch failed a quality test, comparing two treatments, monitoring an environmental indicator over a season, or developing a product. In each case the work is research, and not routine testing, when its aim is to produce knowledge that was not available before and when its steps can be inspected and repeated by someone else. Research methods are the techniques used to collect and analyse evidence, and the study of why one technique suits a question better than another is research methodology.

Characteristics of scientific research

  • Systematic: Each stage follows a planned order, from the question to the report, so that nothing is left to chance or to memory.
  • Empirical: Conclusions rest on observation and measurement, not on opinion or authority. A claim that a drying method lowers moisture is supported by moisture measurements.
  • Objective: The researcher controls bias through design, calibrated instruments, blinded analysis where possible and honest reporting.
  • Controlled: Factors other than the one being studied are held constant, measured or balanced, so that a difference can be traced to its cause.
  • Replicable: The method is described well enough for another trained person to repeat it and obtain a comparable result.
  • Logical: The reasoning from evidence to conclusion can be followed step by step.
  • Cumulative: Each study builds on earlier work, which is why a review of the literature comes before the design.
  • Ethical: The study protects people, animals and the environment and reports truthfully.

The research process

Research follows a cycle of stages, each of which is taught in this unit.

  1. Identify a topic and a problem worth investigating.
  2. Review the existing knowledge to find what is known and what is missing.
  3. State the objectives, the research questions and, where they are suited, the hypotheses.
  4. Build the framework that shows how the variables relate and which theory explains them.
  5. Choose the design, the site, the sample and the instruments.
  6. Obtain ethical approval and permits.
  7. Collect the data using the planned methods and quality control.
  8. Organise and analyse the data.
  9. Interpret the results against the objectives and the literature.
  10. Report the findings, state the conclusions and recommendations, and keep the records.

Stages 1 to 6 are written as the research proposal, which is why this chapter, on preparing for research, leads to proposal writing.

Research methods and research methodology

A method is a specific technique: a titration, a membrane filtration count, an interview guide or a t-test. Methodology is the reasoning that links the problem to the choice of techniques and justifies it. A proposal states the methodology (for example, a controlled laboratory experiment with replicated treatments) and then lists the methods (the standard procedures, the instruments and the statistical tests).

Types of research by purpose

Type Purpose Laboratory example
Basic (pure) Extend understanding with no immediate use in mind Studying how temperature changes the rate of a reaction in a new solvent
Applied Solve a practical problem Testing whether a cheaper reagent gives the same result as the one in use
Exploratory Learn about a topic that is little understood so that later questions can be set Interviewing technicians to find out what causes repeat testing
Descriptive Describe the characteristics of a situation or population Measuring the range of pH in water from a set of springs
Explanatory Explain why something happens or what causes it Testing whether the type of bottle changes the measured pH of stored water samples over one week
Evaluative Judge how well a method, product or programme works Comparing the performance of two disinfectants on bench surfaces
Action research Improve practice in a workplace through repeated cycles of planning, acting and reviewing A laboratory trying a new sample-labelling system and adjusting it over three months

Quantitative, qualitative and mixed approaches

Feature Quantitative Qualitative Mixed
Data Numbers from measurement and counts Words, images and observations Both
Typical question How much, how many, is there a difference How and why, what is the experience Both, one explaining the other
Tools Instruments, structured questionnaires Interviews, observation notes, documents A combination
Analysis Statistics Coding into themes Each kind analysed, then compared
Strength Measures size and tests differences Explains meaning and context Gives both extent and reasons
Limit May miss reasons and context Findings are hard to generalise Needs more time and skill

Most laboratory measurement studies are quantitative. A qualitative strand is added when the question concerns people, such as why a safety procedure is not followed.

Deductive and inductive reasoning

Deductive research starts with a theory or hypothesis and tests it with data. If theory says that a reaction rate rises with temperature, an experiment at several temperatures tests that prediction. Inductive research starts with observations and builds a general explanation from the pattern. Interviews that show the same obstacle to waste segregation in several laboratories lead to a general explanation. Many studies use both: observations suggest a hypothesis and an experiment tests it.

Common research designs

The main designs are introduced here, and the design of a particular study is worked out later in the unit.

Design What it does Laboratory example
Experimental The researcher changes one factor and measures its effect, with controls and replicates Comparing two filter media on turbidity removal under the same conditions
Quasi-experimental Compares groups without full random assignment Comparing water from two sites that differ in treatment
Descriptive survey Measures the characteristics of a group at one time Measuring residue levels in samples collected from several sources
Correlational Examines whether and how strongly two variables are related, without changing either Relating turbidity to coliform counts
Case study Examines one case in depth Tracing the causes of a repeated contamination in one laboratory
Cross-sectional Takes one measurement of many units at one time One-day sampling of many boreholes
Longitudinal Follows the same units over time Measuring a river's dissolved oxygen every month for a year

Classifying a study

Example: a technician proposes "To determine whether heating raw honey above a set temperature increases its hydroxymethylfurfural content". The technician changes temperature and measures content, so the design is experimental. The aim is to explain a cause, so it is explanatory research with a quantitative approach and a deductive reasoning from the idea that heat drives the reaction.

Example: a technician proposes "To describe how laboratory staff record temperature of storage refrigerators and the reasons they give for missing entries". The data are practices and reasons, so the approach is qualitative with a descriptive purpose, using interviews and observation.

Qualities of good research

  • Relevance: The question matters to a laboratory, an industry or a community.
  • Rigour: The design, controls and calculations withstand checking.
  • Transparency: Methods, data and decisions are recorded so that others can examine them.
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🔒4.2 Ethics in research

Research ethics are the standards of conduct that protect the people, animals and environment affected by a study and protect the honesty of the science itself. At the proposal stage ethics is not a formality added at the end: the proposal must show how partic…

🔒4.3 Research topics and study site

A proposal begins with a clear topic and a precise place of study. A topic is a broad area of interest narrowed until it can be investigated with the time, equipment and skills available, and it is expressed in a title that tells the reader what will be measur…

🔒4.4 Research problem

A research problem is a specific difficulty, gap or contradiction that a study sets out to resolve. It is different from a topic, which only names an area ("shelf life of milk"), and from a question, which asks something precise. The problem explains why the s…

🔒4.5 Research objectives

Research objectives are statements of what the study will achieve. They turn the research problem into tasks that can be carried out and checked. Every later part of the study depends on them: the research questions are drawn from them, the design and the meas…

🔒4.6 Designing research questions

A research question is the precise question that the study will answer with data. Objectives say what the study will do, and research questions restate them as questions that point directly at the evidence needed. Where the study tests a prediction, each quest…

🔒4.7 Development of conceptual framework

A conceptual framework is the researcher's own map of the study: a diagram, with a short explanation, that shows the variables to be measured and the relationships expected between them. It is built from the objectives, the research questions and the literatur…

🔒4.8 Establishment of research theoretical framework

A theoretical framework is the established theory, law or model on which a study rests. It explains why the variables are expected to be related, and it gives the researcher a tested basis for the hypotheses, the choice of measurements and the interpretation o…

🔒4.9 Proposal writing procedures

A research proposal is the written plan of a study and the argument for carrying it out. It is read by a supervisor, a review committee or a funder, who must decide whether the problem is worth solving, whether the plan will solve it, whether it can be done in…